BUILDING HYBRID MODELS OF HUMAN CARDIOMYOCYTES: COMBINING SIMULATIONS WITH BIOLOGICAL CELLS IN REAL-TIME
A seminar by prof. Teun P.De Boer (Assistant Professor - Department of Medical Physiology - University Medical Center Utrecht)
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Date: 05 APRIL 2019 from 16:30 to 18:30
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Event location: DEI - Room 2.4, via dell’Università 50, Cesena
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Type: Seminar
An important aspect of our work is the development of real-time simulations of ion channels, the so-called dynamic clamp technique.
The most important application is to simulate cardiac IK1 channels that are missing from stem cell-derived cardiomyocytes (iPSC-CM). By combining the real-time simulated ion channels with human iPSC-CM, we can create a hybrid human cardiomyocyte model that is more similar to human adult cardiomyocytes. The main benefit of this approach is that it enables more specific in vitro testing of drugs that might negatively affect the human heart rhythm.
Current focus is to enhance the throughput of this approach, which resulted in the establishment of a fully automated dynamic clamping system which is integrated with an automated patch clamping device.